全文获取类型
收费全文 | 14041篇 |
免费 | 1269篇 |
国内免费 | 1484篇 |
出版年
2024年 | 14篇 |
2023年 | 174篇 |
2022年 | 202篇 |
2021年 | 621篇 |
2020年 | 517篇 |
2019年 | 604篇 |
2018年 | 561篇 |
2017年 | 454篇 |
2016年 | 608篇 |
2015年 | 918篇 |
2014年 | 1068篇 |
2013年 | 1116篇 |
2012年 | 1450篇 |
2011年 | 1245篇 |
2010年 | 776篇 |
2009年 | 705篇 |
2008年 | 825篇 |
2007年 | 706篇 |
2006年 | 682篇 |
2005年 | 547篇 |
2004年 | 468篇 |
2003年 | 425篇 |
2002年 | 347篇 |
2001年 | 223篇 |
2000年 | 200篇 |
1999年 | 178篇 |
1998年 | 150篇 |
1997年 | 119篇 |
1996年 | 90篇 |
1995年 | 92篇 |
1994年 | 104篇 |
1993年 | 60篇 |
1992年 | 74篇 |
1991年 | 75篇 |
1990年 | 45篇 |
1989年 | 49篇 |
1988年 | 36篇 |
1987年 | 35篇 |
1986年 | 30篇 |
1985年 | 36篇 |
1984年 | 18篇 |
1983年 | 13篇 |
1982年 | 13篇 |
1981年 | 13篇 |
1980年 | 10篇 |
1979年 | 9篇 |
1978年 | 11篇 |
1976年 | 10篇 |
1975年 | 11篇 |
1973年 | 8篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Twelve known and nine new highly oxygenated flavonoid aglycones, some of which exhibit 2′-oxygenation, were isolated from Gutierrezia grandis. 相似文献
992.
993.
Rong Li Xiao‐Ling Tang Shi‐Ying Miao Shu‐Dong Zong Lin‐Fang Wang 《Cell biochemistry and function》2009,27(5):264-268
Sperm associated antigen 8 (SPAG8), a testis‐specific protein produced during male germ cell differentiation, was isolated from a human testis expression library using antibodies found in the serum obtained from an infertile woman. It was found to have a close functional relationship with microtubules. In this study, we generated a stably expressing SPAG8 CHO‐K1 cell line. Immunofluorescence confocal microscopy showed that SPAG8 was concentrated at the microtubule‐organizing center (MTOC) during prophase. As the cells progressed into metaphase, it co‐localized with α‐tubulin on the spindle. In anaphase, it was detected on both astral microtubules and mid‐zone. Following cytokinesis, SPAG8 resumed its localization on the MTOC. Meanwhile, flow cytometry analysis found that SPAG8 prolonged the G2/M phase of CHO‐K1 cells stably expressing SPAG8. Furthermore, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay showed that SPAG8 inhibited the proliferation of the stable cells. SPAG8 might be involved in the regulation of cell cycle by changing the phosphorylation level of Tyr15 on cdc2. These results suggest that SPAG8 might play a role in cell division during spermatogenesis. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
994.
The histological characteristics of the digestive tract and the ultrastructure of mucosal cells of the stomach and intestine of rice field eel, Monopterus albus, are described to provide a basis for future studies on its digestive physiology. The digestive tract of the rice field eel is a long and coiled tube composed of four layers: mucosa, lamina propria‐submucosa, muscularis and serosa. The pharynx and oesophagus mucosa is lined with a stratified epithelium. The stomach includes the cardiac and pyloric portions and the fundus. Many gastric pits are formed by invaginations of the mucosal layer and tubular gastric glands formed by the columnar cells in the fundus. The intestine is separated from the stomach by a loop valve and divided into a proximal portion and a distal portion. The proximal intestinal epithelium consists of columnar cells with microvilli towards the lumen and goblet cells. The enterocytes are joined at the apical surface by the junctional complex, including the evident desmosomas. Numerous lysosomes and some vesicles are evident in the upper cytoplasm of the cells, and a moderate amount of endoplasmic reticulum and lysosomes are scattered in the supranuclear cytoplasm. The epithelium becomes progressively thicker and the folds containing large numbers of goblet cells are fewer and shorter in the distal portion of the intestine. At the ultrastuctural level, the columnar cells of the tubular gastric glands have numerous clear vacuoles and channels. A moderate amount of pepsinogen granules are present in the stomach. The enterocytes of the intestinal mucosa display a moderate amount of endoplasmic reticulum and lysosomes, and long and regular microvilli. 相似文献
995.
The fluorescence of the prulifloxacin (PUFX)–Al(III) system was investigated . Experiments indicated that the fluorescence intensity of prulifloxacin could be greatly enhanced by Al(III) and sensitized by sodium dodecylbenzene sulphonate (SDBS). Accordingly, a sensitive spectrofluorimetric method for the determination of prulifloxacin was established. While excited at 275 nm, the enhanced fluorescence intensity at 412 nm of the system (ΔF) showed a good linear relationship with the concentration of prulifloxacin within the range 4.0 × 10–8–3.0 × 10–6 mol/L. The regression equation was ΔF = 9.83 + 10.8 × 107c (mol/L); the correlation coefficient and detection limit (3σ/k) were 0.99901 and 2.0 × 10–8 mol/L, respectively. The proposed method has been successfully applied to determine prulifloxacin in real pharmaceutical samples. The luminescence mechanism of the system is also discussed in detail. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
996.
997.
Contact Toxicity and Repellency of the Essential Oil from Mentha haplocalyx Briq. against Lasioderma serricorne 下载免费PDF全文
Wen‐Juan Zhang Kai Yang Chun‐Xue You Cheng‐Fang Wang Zhu‐Feng Geng Yang Su Ying Wang Shu‐Shan Du Zhi‐Wei Deng 《化学与生物多样性》2015,12(5):832-839
The chemical composition of the essential oil obtained by hydrodistillation from the aerial parts of Mentha haplocalyx was investigated by GC‐FID and GC/MS analyses. In sum, 23 components, representing 92.88% of the total oil composition, were identified, and the main compounds were found to be menthol (59.71%), menthyl acetate (7.83%), limonene (6.98%), and menthone (4.44%). By bioassay‐guided fractionation (contact toxicity), three compounds were obtained from the essential oil and identified as menthol, menthyl acetate, and limonene. The essential oil and the three isolated compounds exhibited potent contact toxicity against Lasioderma serricorne adults, with LD50 values of 16.5, 7.91, 5.96, and 13.7 μg/adult, respectively. Moreover, the oil and its isolated compounds also exhibited strong repellency against L. serricorne adults. At the lower concentrations tested and at 2 h after exposure, menthol showed even significantly stronger repellency than the positive control DEET. The study revealed that the bioactivity properties of the essential oil can be attributed to the synergistic effects of its diverse major and minor components, which indicates that the M. haplocalyx oil and its isolated compounds have potential for the development as natural insecticides and/or repellents to control insects in stored grains and traditional Chinese medicinal materials. 相似文献
998.
Five new guaiane sesquiterpenes, 1 – 5 , were isolated from the culture broth of the endophytic fungus Xylaria sp. YM 311647, isolated from Azadirachta indica A. Juss . The structures of these compounds were elucidated on the basis of spectroscopic analyses, and their inhibitory activities against five pathogenic fungi were evaluated. All guaiane sesquiterpenes showed moderate or weak antifungal activities in a broth microdilution assay. 相似文献
999.
You‐Min Ying Cui‐Yu Li Yan Chen Jia‐Gui Xiang Ling Fang Jian‐Biao Yao Fa‐Song Wang Ru‐Wei Wang Wei‐Guang Shan Zha‐Jun Zhan 《化学与生物多样性》2015,12(8):1222-1228
Two new triterpenoids, 30‐hydroxylup‐20(29)‐ene 3β‐caffeate ( 1 ) and 24‐nor‐friedelan‐6α,10‐dihydroxy‐1,2‐dioxo‐4,7‐dien‐29‐oic acid ( 2 ), together with eight known compounds 3 – 10 , were isolated from the roots of Celastrus stylosus. The structures of these compounds were elucidated on the basis of spectroscopic analyses. To the best of our knowledge, this represents the first study on the chemical constituents of C. stylosus. The antiproliferative activities of the triterpenoids against six human cancer cell lines (PANC‐1, A549, PC‐3, HepG2, SGC‐7901, and HCCLM3) were evaluated. Compounds 3, 4 , and 10 exhibited comparable activities against PC‐3 and HCCLM3 cell lines as the positive control taxol. 相似文献
1000.
Yu‐Kuang Liao Maël Brossard Dan‐Hua Hsieh Tzu‐Neng Lin Martin D. B. Charlton Shun‐Jen Cheng Chyong‐Hua Chen Ji‐Lin Shen Lung‐Teng Cheng Tung‐Po Hsieh Fang‐I Lai Shou‐Yi Kuo Hao‐Chung Kuo Pavlos G. Savvidis Pavlos G. Lagoudakis 《Liver Transplantation》2015,5(2)
A novel scheme for hybridizing inkjet‐printed thin film Cu(In,Ga)Se2 (CIGS) solar cells with self‐assembled clusters of nanocrystal quantum dots (NQDs), which provides a 10.9% relative enhancement of the photon conversion efficiency (PCE), is demonstrated. A non‐uniform layer of NQD aggregates is deposited between the transparent conductive oxide and a CdS/CIGS p‐n junction using low cost pulsed‐spray deposition. Hybridization significantly improves the external quantum efficiency of the hybrid devices in the absorption range of the NQDs and in the red to near‐IR parts of the spectrum. The low wavelength response enhancement is found to be induced by luminescent down‐shifting (LDS) from the NQD layer, while the increase at longer wavelengths is attributed to internal scattering from NQD aggregates. LDS is demonstrated using time‐resolved spectroscopy, and the morphology of the NQD layer is investigated in fluorescence microscopy and cross‐sectional transmission electron microscopy. The influence of the NQD dose on the PCE of the hybrid devices is investigated and an optimum value is obtained. The low costs and limited material consumptions associated with pulsed‐spray deposition make these flexible hybrid devices promising candidates to help push thin‐film photovoltaic technology towards grid parity. 相似文献